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當前位置:首頁  >  產(chǎn)品中心  >  細胞生物學  >  細胞系  >  T25人小細胞肺癌細胞 NCI-H446 H446 CLH1269
型號:T25

人小細胞肺癌細胞 NCI-H446 H446 CLH1269

描述:人小細胞肺癌細胞 NCI-H446 [H446] T25/瓶 CLH1269

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    2025-07-09
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品牌CellWorld規(guī)格T25/瓶
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應用領域生物產(chǎn)業(yè)

HC1269  NCI-H446 [H446]細胞專用培養(yǎng)基

1640+10% FBS+1% P/S


NCI-H446 [H446](人小細胞肺癌細胞)基本介紹  

細胞簡介: NCI-H446細胞于1982年由D.Carney、A.F.Gazdar及其同事從一名61歲白人男性小細胞肺癌患者的胸腔液中分離出來,原始腫瘤的形態(tài)不是SCLC的特征。NCI-H446細胞是SCLC的生化和形態(tài)學變體,表達神經(jīng)元特異性烯醇化酶和肌酸激酶的腦同工酶。NCI-H446細胞檢測到L-DOPA脫羧酶、蛙皮素、加壓素、催產(chǎn)素或胃泌素釋放肽的水平。與正常細胞相比,NCI-H446細胞C-myc DNA序列擴增約20倍,C-myc RNA增加15倍。NCI-H446細胞最初在補充有10nM氫化可的松、0.005mg/mL胰島素、0.01mg/mL轉鐵蛋白、10nM 17-β-雌二醇和30nM亞硒suan na 的無血清RPMI 1640培養(yǎng)基中繁殖。NCI-H446細胞在裸鼠中形成具有非典型SCLC組織學的可移植腫瘤。

細胞貨號: CLH1269

細胞名稱: NCI-H446 [H446](人小細胞肺癌細胞)

細胞別稱: H446; H-446; NCI-446; NCIH446

來       源: 小細胞肺癌;胸腔積液轉移;男性;61歲

細胞形態(tài): 上皮細胞樣 細胞類型 腫瘤細胞

生長特性: 貼壁細胞 收錄ATCC

培養(yǎng)條件: 1640+10% FBS+1% P/S 培養(yǎng)環(huán)境 37℃;5% CO2;飽和濕度


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